活性Cas12 (LbCpf1)核酸酶
大肠杆菌表达的重组蛋白
Catelog No. C12CR-E241H
Catalog No. Pack Size Price
C12CR-E241H-5050 pmolContect Us
C12CR-E241H-100100 pmolContect Us
C12CR-E241H-200200 pmolContect Us
C12CR-E241H-10001000 pmolContect Us
C12CR-E241H-BULKBULKContect Us

Document


  • SDS

  • Data Sheet
Overview CRISPR(成簇的有规律的间隔短回文重复)及其CRISPR相关(Cas)蛋白构成细菌的适应性免疫系统 (1-2)。该系统已经过重新设计,作为基因组编辑工具用于基于 RNA 的治疗手段的产品开发(3)。快速敏感的敏感的体外诊断工具是成功治疗患者和遏制疾病传播的关键环节。一些基于 CRISPR-Cas12 的 SARS-CoV-2 检测方法具有已成功开发 (4,5)。 CRISPR/Cas12 (LbCpf1) 是 来自毛螺菌科细菌Cas12合成构建体。本产品是大肠杆菌表达的LbCpf1Cas12, 在C-端带有His-tag.
Genbank Number ATB19154
Storage and Stability 产品储存在-70摄氏度。为了最佳的存储,离心后将目标分装成少量,并在推荐的温度下存储。为了获得最佳性能,应避免重复处理和多次冻融循环
Purity
Molecular weight 130KDa
Specific Activity
References
  1. Horvath, P., et al. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
  2. Morange, M. What history tells us XXXVII. CRISPR-Cas: The disconvery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
  3. Gier, R.A., et al. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 2020, 11(1):1-9.
  4. Broughton JP, et al: Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv doi: https://doi.org/10.1101/ 2020.03.06.20032334.
  5. Lucia C, et al: An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12. bioRxiv. doi: https://doi.org/10.1101/ 2020.02.29.971127.